Investigate significant genes in central metabolism

Interactive plot instructions:

Upper Left panel (Brush Selection)	Right Panel (Pan/Zoom; Legend Selection)
Click+drag mouse to highlight a region of points Click empty space to clear selection	Click+drag mouse to pan around the plot Use scroll wheel to zoom Hover over points for more information about each gene Click Pathways in the legend to highlight genes in the selected pathway Hold SHIFT and Click pathways in the legend to select multiple Click anywhere in legend to clear selection
Lower Left Panel (Selection response)
Bar lengths correpsond to number of pathway genes brush-selected in upper left panel

Figure description

Volcano plot comparing differential gene expression between limited methane conditions (500ppm or 1000ppm) and the baseline 2.5% (v/v) methane growth condition.

• x-axis: magnitude of the log₂ fold change
• y-axis: differential expression p-value (DESeq2, plotted as inverse log₁₀ for visual clarity).

The upper left and large right panel contain the same data points but provide different interactions (brush select vs. pan + zoom). The lower left panel is a bar chart showing the count of selected genes annotated in each pathway (note: the count is doubled since each gene exists once in the 500ppm (square) and once in the 1000ppm (circle) dataset). The length of the bars dynamically respond to the brush selection made in the upper left panel.