Tradeoff between gene count and TPM expression for different top gene sets depending on the n% threshold. Each point denotes a top gene set and shows the total count of all genes in the set on the x-axis versus the minimum gene’s mean TPM value on the y-axis. For small values of n, the mean TPM expression of the weakest gene in the top set increases, while the total count of genes in the top set decreases, and vice versa. Filled circles represent all genes that qualify as the top n% across all conditions. Open circles represent filtered versions of the same set, where any gene predicted to be inside an operon (any except the first gene) has been excluded.